Aptamer-Based Extraction of Ergot Alkaloids from Ergot Contaminated Rye Feed

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Aptamer-Based Extraction of Ergot Alkaloids from Ergot Contaminated Rye Feed

Ergot alkaloids are mycotoxins which can be found in food based on cereal-crops, due to a contamination of plants by fungi of the genus Claviceps. The ingestion of ergot contaminated cereal crops can lead to a severe poisoning known as ergotism. For food and feed safety purposes, the extraction of ergot alkaloids from ergot contaminated flour was investigated. For the specific recognition of er...

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Analysis of Ergot Alkaloids

The principles and application of established and newer methods for the quantitative and semi-quantitative determination of ergot alkaloids in food, feed, plant materials and animal tissues are reviewed. The techniques of sampling, extraction, clean-up, detection, quantification and validation are described. The major procedures for ergot alkaloid analysis comprise liquid chromatography with ta...

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Synthesis of Ergot Alkaloids from Tryptophan

The first total syntheses of lysergine and setoclavine are described, as well as a new and efficient synthesis of lysergic acid. The starting material for these syntheses is tryptophan, protected as its dihydro, dibenzoyl derivative. This material is dehydrated to the corresponding azlactone, which undergoes stereoselective intramolecular Friedel-Crafts acylation to give a tricyclic ketone inte...

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Biosynthetic Pathways of Ergot Alkaloids

Ergot alkaloids are nitrogen-containing natural products belonging to indole alkaloids. The best known producers are fungi of the phylum Ascomycota, e.g., Claviceps, Epichloë, Penicillium and Aspergillus species. According to their structures, ergot alkaloids can be divided into three groups: clavines, lysergic acid amides and peptides (ergopeptines). All of them share the first biosynthetic st...

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Screening Isolates for Ergot Alkaloids

A procedure was developed to isolate and determine ergot alkaloid production by Acremonium coenophialum, the endophytic fungus of tall fescue. The procedure established that macerated leaf sheath or pith from inRorescence stem placed either in a liquid medium or on a corn meal-malt extract agar medium produced isolated mycelium and characteristic conidia within a 3to 3.5-week period. Once isola...

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ژورنال

عنوان ژورنال: Advances in Bioscience and Biotechnology

سال: 2014

ISSN: 2156-8456,2156-8502

DOI: 10.4236/abb.2014.58082